| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 591 | Methylorubrum populi | TTCGATCCGGAACTCTGCC | TTCGCTTGCGGTCGAACA | 59.48 | 60.28 | 163 | 64 |
100.00%
|
100.00%
|
| Primer 592 | Methylorubrum populi | TGTTGGAACTGCTGCCCA | AGGCTGCGCAACTTGGT | 59.40 | 59.84 | 274 | 64 |
100.00%
|
100.00%
|
| Primer 593 | Methylorubrum populi | AGCGTGTTCCGGACAACA | TCGATCCACTCCTCCAGCA | 59.50 | 60.00 | 163 | 64 |
100.00%
|
100.00%
|
| Primer 594 | Methylorubrum populi | AGCGTGTTCCGGACAACA | TCGGACTGGTCGATCCACT | 59.50 | 60.00 | 172 | 64 |
100.00%
|
100.00%
|
| Primer 595 | Methylorubrum populi | TGACGCGAGCCTTCATCT | TCAGGGTGAGTTCGTCGGA | 58.71 | 60.23 | 171 | 63 |
100.00%
|
100.00%
|
| Primer 596 | Methylorubrum populi | TACGTCCCGTGCAAGCTCT | GCACGGTTTCGAGCTTCTTC | 61.27 | 59.84 | 265 | 63 |
100.00%
|
100.00%
|
| Primer 597 | Methylorubrum populi | TCCTACGAGGTCGGGTTCT | AGTGCAGCCATGTCAGCA | 59.31 | 59.57 | 283 | 63 |
100.00%
|
100.00%
|
| Primer 598 | Methylorubrum populi | ATGCTGCGACCCGATTCT | ATTGTGCGAGACGAGGAGC | 59.10 | 60.15 | 191 | 63 |
100.00%
|
100.00%
|
| Primer 599 | Methylorubrum populi | TGGCGATGGTGTTCGTCT | AACAGGAAGGCACGCTGT | 58.94 | 59.49 | 202 | 63 |
100.00%
|
100.00%
|
| Primer 600 | Methylorubrum populi | CTGGCGATGGTGTTCGTCT | AACAGGAAGGCACGCTGT | 60.08 | 59.49 | 203 | 63 |
100.00%
|
100.00%
|